Journal article
Targeting Ability of Affibody-Functionalized Particles Is Enhanced by Albumin but Inhibited by Serum Coronas
Q Dai, Y Yan, J Guo, M Björnmalm, J Cui, H Sun, F Caruso
ACS Macro Letters | Published : 2015
Abstract
Protein coronas formed on engineered particles can alter their targeting ability as they enter biological environments. Here, we engineer polymer-coated silica particles and investigate the influence of protein coronas derived from various sources. The particles were functionalized with a small antibody-mimetic ligand (affibody), and their targeting ability to cancer cells in the presence of protein coronas was determined. Protein coronas derived from human serum showed a dramatic inhibition of specific particle-cell association (from ∼70 to ∼7%), whereas the most abundant protein in human serum - human serum albumin - enhanced the specific association of functionalized particles to SK-OV-3 ..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
This research was conducted and funded by the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036). This work was also supported by the Australian Research Council under the Australian Laureate Fellowship (F.C., FL120100030) and Discovery Early Career Researcher Award (Y.Y., DE130100488) schemes. We acknowledge funding from the Australian Government through an International Postgraduate Research Scholarship and an Australian Postgraduate Award (M.B.), as well as The University of Melbourne through a Melbourne International Research Scholarship (Q.D.). This work was performed in part at the Materials Characterisation and Fabrication Platform (MCFP) at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility (ANFF).